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EFFECT OF UNREACTED RESIN MONOMES ON THE ATIVITY OF CARIOGENIC BACTERIA

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Abstract

º» ¿¬±¸´Â º¹ÇÕ·¹Áø¿¡¼­ À¯ÃâµÈ ¼ö¿ë¼º ¹Ì¹ÝÀÀ´Ü·®Ã¼°¡ Ä¡¾Æ ¿ì½Ä¹ß»ý ¿øÀαÕÀÎ S. mutans¿Í S. sobrinusÀÇ Áõ½Ä°ú ºñ¼ö¿ë¼º glucanÀÇ Çü¼º´É¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» Æò°¡ÇÏ°íÀÚ ±¤ÁßÇÕ ·¹ÁøÀü»öü¸¦ 10ºÐ, 1½Ã°£, 12½Ã°£, 24½Ã°£ ¹°¿¡ ħÀá½ÃÄÑ ·¹ÁøÈ­Çй°À» ¿ëÃâÇÑ ÈÄ 1/2, 1/4, 1/8ÀÇ ³óµµ·Î ÷°¡ÇÑ BHI broth ¶Ç´Â BHI-YS¿¡¼­ ¼¼±ÕÀ» 24½Ã°£ ¹è¾çÇÑ ÈÄ, Èí±¤µµ¸¦ ÃøÁ¤ÇÏ¿© ´ÙÀ½°ú °°Àº °á°ú¸¦ ¾ò¾ú´Ù.
1. S. mutansÀÇ ¼ºÀå·üÀº 10ºÐ ¿ëÃâ¾×ÀÇ 1/4³óµµ¿¡¼­ ¼ºÀåÁ¶Àý±º¿¡ ºñÇØ ÇöÀúÈ÷ °¨¼ÒµÈ ¹Ý¸é 1/2, 1/8³óµµ¿¡¼­´Â Áõ°¡ÇÏ´Â °æÇâÀ» º¸¿´À¸¸ç, 1½Ã°£ ¿ëÃâ¾×Àº S. mutansÀÇ ¼ºÀåÀ» ÃËÁøÇÏ¿´À¸¸ç, ƯÈ÷ 1/2, 1/4³óµµ¿¡¼­ ¼ºÀå·üÀÌ ³ô¾Ò´Ù. ¶ÇÇÑ 12½Ã°£, 24½Ã°£ ¿ëÃâ¾×À» S. mutansÀÇ ¼ºÀå¿¡ ¿µÇâÀ» ÁÖÄ¡ ¾Ê¾Ò´Ù.
2. S. sobrinusÀÇ ¼ºÀå·üÀº 10ºÐ ¿ëÃâ¾×ÀÇ 1/4³óµµ¿¡¼­´Â ÇöÀúÈ÷ ¾ïÀçµÇ¾úÀ¸³ª, 1½Ã°£, 12½Ã°£, 24½Ã°£ ¿ëÃâ¾×Àº ¸ðµç ³óµµ¿¡¼­ S. sobrinusÀÇ ¼ºÀå¿¡ ¿µÇâÀ» ÁÖÁö ¾Ê¾Ò´Ù.
3. ·¹Áø¿ëÃâ¾×Àº ¼ºÀåÁ¶Àý±º¿¡ ºñÇØ S. mutansÀÇ ÃѼºÀåÀ» ÃËÁøÇÏ¿´À¸³ª S. sobrinusÀÇ ÃѼºÀåÀº ¾ïÁ¦ÇÏ¿´´Ù.
4. S. mutansÀÇ ºñ¼ö¿ë¼º glucanÇü¼º´ÉÀº ¼ºÀåÁ¶Àý±º¿¡ ºñÇØ ÃËÁøµÇ¾úÀ¸¸ç, ƯÈ÷ 10ºÐ ¿ëÃâ¾×ÀÇ 1/4³óµµ¿¡¼­ ÇöÀúÇÏ¿´À¸¸ç, ¸ðµç¿ëÃâ¾×ÀÇ 1/8³óµµ¿¡¼­´Â glucanÀÇ Çü¼º´ÉÀº °¨¼ÒÇÏ¿´´Ù.
5. S. sobrinusÀÇ ºñ¼ö¿ë¼º glucanÀÇ Çü¼º´ÉÀº 10ºÐ¿ëÃâ¾×ÀÇ 1/4 ³óµµ¸¦ Á¦¿ÜÇÏ°í´Â ¼ºÀå´ëÁ¶±º¿¡ ºñÇØ °¨¼ÒÇÏ¿´´Ù.
6. S. mutansÀÇ ºñ¼ö¿ë¼º glucanÀÇ ÃÑÇü¼º·®Àº S. sobrinus¿¡¼­º¸´Ù ¾à 3¹è Á¤µµ ³ô¾Ò´Ù.
The aim of this study was to investigate the effect of composite resin components on proliferation and glucan synthesis by cariogenic bacteria, Streptococcus mutans and Streptococcus sobrinus. Light curing pit and fissure sealant was chosen for evluation. Specimens were eluted in deionized water for 10 minutes, 1, 12, and 24 hours. Extracts of specimens were diluted into 1/2, 1/4, and 1/8 with addition of BHI broth and BHI-YS. Bacteria were cultured in media included eluted components, and measured optical density(A600).
The following results were obtained
1. 1/4 concentration of elutes for 10 minutes significantly inhibited the proliferation of S. mutans, whereas 1/2, 1/8 concentration of elutes stimulated it. Also, exacts, especially 1/2, 1/4 concentration, for 1 hours stimulated it. But exacts for 12, 24 hours had not effects on the proliferation of S. mutans.
2. 1/4 concentration of elutes for 10 minutes inhibited growth of S. sobrinus, whereas extracts for 1, 12, 24 hours had not effects on the proliferation of S. sobrinuss.
3. Extracts from composite resin stimulated total growth of S. mutans more than growth control group, where¡þas inhibited it of S. sobrinus.
4. Extracts from composite resin, especially 1/4 concentration of it for 10 minutes increased the formation of water insoluble glucan of S. mutans. But elutes for 1. 12, 24 hours, and 1/8 concentration of it for 10 min¡þutes inhibited it.
5. Except 1/4 concentration of elutes for 10 minutes, extracts decreased the formation of water insoluble glu¡þcan of S. sobrinus.
6. Total amount of formated glucan was 3-fold higher in S. mutans than in S. sobrinus.

Å°¿öµå

S. mutans;S. sobrius;¹Ì¹ÝÀÀ ´Ü·®Ã¼;¼ö¿ë¼º ±Û·çĵ;Unreacted resin monomer;Water insoluble glucan

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